Journal: Pharmaceuticals

Article Title: Novel Anti-Enterovirus A71 Compounds Discovered by Repositioning Antivirals from the Open-Source MMV Pandemic Response Box

doi: 10.3390/ph17060785

Figure Lengend Snippet: Evaluation of potential anti-EV-A71 activity of the selected 18 antiviral compounds from the MMV Pandemic Response Box. RD cell monolayers were infected with EV-A71 at 1 M.O.I. by adsorbing with the virus inoculum for 1 h and then incubated with fresh medium containing 1 μM of the tested compounds for 24 h. The D-A7 (rupintrivir) and D-H7 (T-1106) compounds served as positive anti-EV-A71 activity control. The infected RD cell monolayers incubated with cell culture medium supplemented with DMSO (infDMSO) served as negative anti-EV-A71 activity control. Uninfected RD cells incubated with cell culture medium alone (DMEM) and DMSO served as maximum % cell survival and vehicle control, respectively. The SRB assay determined % cells rescued from EV-A71-induced cytopathic effect (CPE) and was calculated for % cell survival relative to the maximum % cell survival control. The % cell survival values are derived from three independent experiments and presented in Box-and-whisker and dot plots as described in . The dashed line represents the cut-off point relative to the positive anti-EV-A71 activity control (D-H7).

Article Snippet: Rhabdomyosarcoma (RD) cells (JCRB9072) were purchased from the Japanese Collection of Research Bioresources Cell Bank and grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, New York, NY, USA) supplemented with 1× GlutaMAX (Gibco, New York, NY, USA), 10% heat-inactivated fetal bovine serum (HyClone, Waltham, MA, USA), L-glutamine, and penicillin/streptomycin antibiotics (Gibco, New York, NY, USA), namely completed DMEM.

Techniques: Activity Assay, Infection, Virus, Incubation, Control, Cell Culture, Sulforhodamine B Assay, Derivative Assay, Whisker Assay